[ccp4bb] poor SelMet Incorpoation

2014-07-03 Thread Maher Alayyoubi
Hi Everyone, Can someone please help me figure out what's wrong with my SelMet incorporation protocol. My protein of interest is 500 amino acids with 20 of these being Methionines. However mass spec data show that only two of these 20 Methionine sites are partially labelled with SelMet. The

[ccp4bb] Selenomethionine crystals

2014-07-01 Thread Maher Alayyoubi
Hi everyone, Would anyone know for how long Selenomethionine derivative crystals are good if kept in plate at RT. In other words, would SE loose its scattering properties due to oxidation over time? I have SElmet crystals that have been lying in a plate for 2 months by now so I was wondering if

[ccp4bb] Acorn, CRANK

2014-06-30 Thread Maher Alayyoubi
Hi everyone, I have two questions: 1- I was trying to run the program Acorn, on a SAD dataset (Se derivative) that was scaled in scalepack/HKL2000. converted to .mtz using scalepack2mtz, then edited in REVISE and Ecalc. But when I ran Acorn it gives me the error message Segmentation fault. Does

[ccp4bb] crystallographic association

2013-07-08 Thread Maher Alayyoubi
Hi everyone, I just wanted to ask if there is a professional association for crystallographers in the US. When I googled it I came across The American Crystallographic Association. Is that the only one? Thank you, Maher

[ccp4bb] symmetry related molecules

2012-04-12 Thread Maher Alayyoubi
Hi guys, Does any body know if there is a software that would analyse the interface between a protein molecule and its symmetry related molecules in the crystal structure? Thank you, Maher

[ccp4bb] Pymol question

2011-05-25 Thread Maher Alayyoubi
Hi everyone, I have two questions: 1- Does anybody know what are the units on the display ruler after you calculate the vaccum electrostatics using pymol? 2- What are the default kT/e values used by pymol? Thank you, Maher

Re: [ccp4bb] Map correlation coefficient

2011-04-21 Thread Maher Alayyoubi
? Thank you Maher On Wed, Feb 23, 2011 at 11:40 PM, Maher Alayyoubi maher.alayyo...@gmail.com wrote: Hi Everybody, I am a new user on the ccp4 bulletin, I have a question on how to calculate the map correlation coefficient using Overlapamp or any other program? Thank You, Maher

[ccp4bb] Map correlation coefficient

2011-02-24 Thread Maher Alayyoubi
Hi Everybody, I am a new user on the ccp4 bulletin, I have a question on how to calculate the map correlation coefficient using Overlapamp or any other program? Thank You, Maher