Dear all,
We have two funded postdoctoral positions available to investigate
mitochondrial gene expression and genome-segregation mechanisms primarily using
cryo-EM but also biochemistry and macromolecular crystallography (MX). We are
located in the newly-built Biomedicum on the northern campu
Hi Tom,
For acidic side-chains, these kind of interactions are described by Maria
Flocco and Sherry Mowbray in:
"Strange bedfellows: interactions between acidic side-chains in proteins." J.
Mol. Biol. (1995) 254, 96-105.
Best regards,
Martin
On Mar 28, 2014, at 12:11 AM, Tom Peat wrote:
>
Two Postdoctoral Positions – Structural Biochemistry of Mitochondrial RNA
Biogenesis
Successful candidates will participate in all steps involved in structure
determination of DNA/RNA/protein complexes relevant to mitochondrial RNA
biogenesis in the laboratory of Martin Hallberg at the Karolinsk
Raji, we use the 50 ml Cornings up to 25K RCF in our F13S-14x50cy rotor without
leakage so it could be a batch specific problem with your Corning tubes. Try to
talk to Piramoon or one their resellers (Thermo?) in your country if they have
some batches of 50 ml tubes in stock that they can guaran
You can try STRIDE2PyMOL:
http://pldserver1.biochem.queensu.ca/~rlc/work/pymol/stride_ss.py
/Martin
On Jan 24, 2012, at 9:57 PM, Ed Pozharski wrote:
> I am looking for a program/server that would determine secondary
> structure from a pdb file and then output a new pdb file with
> HELIX/SHEET r
Hi,
With the electronic Biohit 8 channel pipette you can set up 3x96 drops in 5
minutes. Lowest volume is 0.2 ul. We have used this system for a couple of
years with RNase free filter tips for RNA-protein complexes. With the recent
arrival of a shared Mosquito we have used the Biohit less but it
Dear Phil,
Is the twin fraction varying? Can you correlate the variation of twin fraction
with variations in crystal morphology or handling?
With constant perfect twinning you could try MIR using the phase determination
method of Yates & Rees (Acta Cryst. (1987). A43, 30-36). I can't access the
Dear Anita,
Sometimes the protein of interest has a relatively strong inherent binding
affinity to the IMAC
column. Have you tried to bind the cleavage reaction to an IMAC column
and then elute using a shallow imidazole gradient?
In fact, Porath developed IMAC chromatography as a tool for prote
Hi,
I'll second Israels's comment. Since the yield per coupling in synthesis is
lower for RNA than for DNA it gets really expensive over 30-35 nucleotides.
However, you can stitch together several 30 nt oligos using either T4 RNA
ligase or T4 DNA ligase (with a DNA splint).
Regarding suppliers
