Dear All,
I have searched the archives and would like more information about
reproducing robot tray hits using 24-well hand trays. I reproducibly get
crystals when I use small volumes (0.5 ul) in 3-well intelliplates but only
precipitate in 1-2 ul sitting drops in 24-well hand plates.
What parame
I am trying to myristoylate a protein (via the N-terminal glycine) using
purified recombinant N-myristoyltransferase and myristoyl-CoA. However I am
having problems with solubility. I can solubilize the unmyristoylated
protein in 250 mM NaCl and 25 mM HEPES at pH 8.0.
I wonder if anyone on this li
