Thank you, everyone!
Francesca
From: Enrico Stura [est...@cea.fr]
Sent: Tuesday, February 17, 2015 3:00 AM
To: CCP4BB@jiscmail.ac.uk; Mattiroli,Francesca
Subject: Re: [ccp4bb] how to reduce protein solubility
Francesca,
The most common failure is to have
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions). The proteins are expressed in insect cells
and going to higher concentration is not easily