Re: [ccp4bb] how to reduce protein solubility

2015-02-17 Thread Mattiroli,Francesca
Thank you, everyone! Francesca From: Enrico Stura [est...@cea.fr] Sent: Tuesday, February 17, 2015 3:00 AM To: CCP4BB@jiscmail.ac.uk; Mattiroli,Francesca Subject: Re: [ccp4bb] how to reduce protein solubility Francesca, The most common failure is to have

[ccp4bb] how to reduce protein solubility

2015-02-16 Thread Mattiroli,Francesca
Hi all, I am struggling with a protein complex that is too soluble. I have reached about 20 mg/ml but I still observe very little precipitation (clear drops in 90-95% of the tested conditions). The proteins are expressed in insect cells and going to higher concentration is not easily