[ccp4bb] occupancy factors for alternate conformations and alternate ligands

Dear colleagues, If I have alternate ligands overlapping in the same electron density or alternate conformations of the protein chain overlapping in the same electron density, how accurate are the occupancy factors? (Res 2A - temp factors are fine) Thanks, Mike Colaneri ###

[ccp4bb] crystals mounted in microRT capillaries

Dear all, how can one mail crystals mounted in loops using the microRT capillaries of Mitegen? Thank you. Mike Colaneri To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/web

[ccp4bb] occupancy refinement

Dear all, Is it reasonable to refine occupancy in phenix at 2.2 A resolution? Thank you. Mike Colaneri

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Dear all, Is it reasonable to refine occupancy in phenix at 2.2 A resolution? Thank you. Mike Colaneri

[ccp4bb] Akta Prime

Dear all, We have an AktaPrime and GE Lifesciences stop servicing these instruments because they are getting old. Does anyone know of a third party company that gives contracts to maintain these instruments? Thank you. Mike Colaneri

[ccp4bb] macbook pro 13"

Dear colleagues, We want to buy a macbook pro 13". It has Nvidia Geforce 320M graphics. Has anyone tried this computer and graphics with coot, O and Pymol and what is the performance? We would appreciate all suggestions. Mike Colaneri

[ccp4bb] Hi prep column from GE

We have a hi prep SP column from GE. We try to load ~14 mg protein but all goes to FT. We lowered the pH and changed the buffer but no luck. We would appreciate all suggestions. Mike Colaneri

[ccp4bb] progrant to calculate error in derived parameters

Dear colleagues, Is there a program to calculate error in bond ables or other angles between coordinates from Luzzati error? Thanks. Mike

[ccp4bb] high pI protein

Hi all, We have a protein with high pI and we can only dissolve it at pH 10.2. Is this a reasonable pH to work with? Should we try to bind something to it to decrease the pH we are working with? We would appreciate all suggestions. Mike Colaneri

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Hi all, We have a protein with high pI and we can only dissolve it at pH 10.2. Is this a reasonable pH to work with? Should we try to bind something to it to decrease the pH we are working with? We would appreciate all suggestions. Mike Colaneri

[ccp4bb] question on scalepack

Dear colleagues, How do I get from scalepack the average I/sigma for the higher resolution shell? Mike Colaneri

[ccp4bb] analytical ultracentrifugation

Dear colleagues, does anyone know of a faciltiy for analytical ultracentrifugation? Thanks. Mike Colaneri

[ccp4bb] DLS and SEC-LS facility

Dear colleagues, I am looking for a facility where I can send samples for DLS and/or SEC-LS on a protein without long wait. Do you know of a company or academic facilty that does that. Thanks. Mike Colaneri

[ccp4bb] question on how crystals form

Dear colleagues, I have a structure with two identical dimers per asymmetric unit. If the dimers are identical in different crystalline environments and in different crystal forms they should be particularly stable. How does such a stable dimer crystallize? Is it necessay to pre-exist in the sp

[ccp4bb] hydrodynamic radius from atomic coordinates

Dear colleagues, Does anyone know of a program to calculate hydrodynamic radius of a protein from atomic coordinates? Thanks. Mike Colaneri

[ccp4bb] extra line of stain in our gel

Hello, When we develop our gel we recently keep getting a horizontal line of stain at 5 mol weight in all lanes of our gel. (This is not a feature of interest except that the protein that we are interested happens to be 5 mol weight). I would appreciate any ideas on how we can get rid o

[ccp4bb] High Wilson B

Dear colleagues, I have a B of 75A**2 from Wilson statistics 4.7 to 3 A res, good straight line. Has anyone seen a B so high in Wilson statistics? ( I understand that it is best to have higher res but I do not). Thanks. Mike Colaneri

[ccp4bb] can glucose covalently bond to the guanidino nitrogen of arginine?

Dear colleagues, Has anyone seen a glucose covalently bonded to the guanidino nitrogens of Arginine via a glycosidic bond? Thanks. Mike Colaneri

[ccp4bb] high temperature factors

Hello, We have a structure that consists of two molecules per asymmetric unit, each molecule is a hexamer. We solved the structure with molecular replacement, R=22%, Rfree=27%. One hexamer has temp. factors (with bdomain.inp of cns) around 50A2. The other has temp factors >60A2 with two subunits