Re: [ccp4bb] Microfocus beamlines (fast-tracks)

2018-10-05 Thread Morgan Milton
Hi Mariana, Where are you located? Sector 23 at Argonne National Labs has a minibeam that goes down to 5 um. APS does rapid request proposals, so you may be able to get beamtime before the end of the year. Morgan E. Milton, PhD On Fri, Oct 5, 2018 at 9:47 AM Mariana Morais wrote: > Dear CCP

Re: [ccp4bb] Off topic: Flourescence anisotropy measurement

2017-07-21 Thread Morgan Milton
Completely agree, you need a higher DNA concentration. We have had luck with 10 nM DNA. Also, bubbles have a HUGE impact on how the fluorescent signal is measured. Make sure you spin your plates down (assuming you are using them) to remove any bubbles. We just had an undergrad read his anisotropy