Hi Nicola,
One way to do it is to dilute your protein, 10-100 times, and add zinc (also
diluted), then concentrate.
Here is the procedure we used some time ago for a zinc-binding protein:
“S100A12 was diluted to 0.1 mg/ml-1 (approximately 10 mM) in a buffer
containing 20 mM Tris-HCl pH 7.5, 200
Dear All,
I am trying to run shelx c/d/e from ccp4 interface (latest updated version of
CCP4). The program terminates with message:
"Arrays too small to generate reflection data -try -L1". (Which, as I
understand, means I have too many reflections, and that one way around the
problem
is to use
Dear All,
I have a problem with building DNA in COOT - looks like mutation to thymine
doesn’t work?
May be I am doing something wrong?
Thanks,
Olga
Dr Olga Moroz
Structural Biology Laboratory,
University of York,
YORK YO10 5DD
UK
tel
:)
Paul Emsley replied to my thymine question, asking me if I was sure I was
building DNA - thank you Paul!
However, in my defence, the option "other modelling tools > build nucleic acid"
doesn’t ask if I’d like DNA or RNa, just builds RNA.
Best wishes,
Olga
Dr Olga Moroz
Struc
Dear All,
It looks like our old Hydra died of old age, and new Hydras seem quite
expensive. We are now looking for an optimal way to transfer crystallisation
screens
from the deep well blocks into crystallisation plates. We were told
Liquidator96 from Rainin, 5-200mkl worked well and was very e
Many thanks to all who replied!
I got emails from people who are also interested, asking to summarise the
results, so here is what I’ve got so far:
1) Liquidator 96 200mkl from Rainin. Everyone agrees it works well and is easy
to use, but tips from Rainin are required.
2) Integra VIAFLO 96. Ti
Same with me, I have Mac OS 10.11.6 (El CApitan).
I couldn’t add terminal residues, then went three CCP4 updates back, it got OK,
but I still can’t add OXT.
Olga
> On 14 Dec 2016, at 04:51, Jobichen Chacko wrote:
>
> Dear All,
> Any solution to this problem.
> I am also encountering this iss
MMS (microseed matrix screening) into several screens, as Patrick suggested
earlier, would be the first choice for me.
Works very often.
Good luck!
Olga
> On 12 Jul 2017, at 08:48, Frank von Delft wrote:
>
> The point I was failing to make: reducing either protein or precipitant
> concentr
MMS often works well for us too!
Best wishes,
Olga
> On 5 Jun 2018, at 19:24, Artem Evdokimov wrote:
>
> Janet, Patrick, and others beat me to it :)
>
> We have tremendous luck with MMS in cases like this.
>
> I would also suggest looking at 'atypical' additives - the word atypical is
> re
Hi everyone,
I always thought it is better to truncate so that biologists looking at the
structures are not misled?
Not sure it the best aproach though...
Olga
> On 10 Mar 2023, at 02:45, Bernhard Lechtenberg
> <968307750321-dmarc-requ...@jiscmail.ac.uk> wrote:
>
> Hi Rhys,
>
> I am a
Dear All,
Does anyone use Jansci PS256 imager? And if yes, what do you think about
it? Is it reliable, easy to use, or has some problems? We are thinking of
buying it to replace our Rigaku Minstrel, which has been out of action for
more than a year...
Thanks a lot!
Olga
Dr Olga Moroz
York
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