Re: [ccp4bb] Adding Zinc to Protein

Hi Nicola, One way to do it is to dilute your protein, 10-100 times, and add zinc (also diluted), then concentrate. Here is the procedure we used some time ago for a zinc-binding protein: “S100A12 was diluted to 0.1 mg/ml-1 (approximately 10 mM) in a buffer containing 20 mM Tris-HCl pH 7.5, 200

[ccp4bb] shelx c/d/e question

Dear All, I am trying to run shelx c/d/e from ccp4 interface (latest updated version of CCP4). The program terminates with message: "Arrays too small to generate reflection data -try -L1". (Which, as I understand, means I have too many reflections, and that one way around the problem is to use

[ccp4bb] can't mutate nucleotides to thymine in Coot

Dear All, I have a problem with building DNA in COOT - looks like mutation to thymine doesn’t work? May be I am doing something wrong? Thanks, Olga Dr Olga Moroz Structural Biology Laboratory, University of York, YORK YO10 5DD UK tel

[ccp4bb] can't mutate nucleotides to thymine in Coot - update

:) Paul Emsley replied to my thymine question, asking me if I was sure I was building DNA - thank you Paul! However, in my defence, the option "other modelling tools > build nucleic acid" doesn’t ask if I’d like DNA or RNa, just builds RNA. Best wishes, Olga Dr Olga Moroz Struc

[ccp4bb] advice on screen dispensing?

Dear All, It looks like our old Hydra died of old age, and new Hydras seem quite expensive. We are now looking for an optimal way to transfer crystallisation screens from the deep well blocks into crystallisation plates. We were told Liquidator96 from Rainin, 5-200mkl worked well and was very e

Re: [ccp4bb] advice on screen dispensing?

Many thanks to all who replied! I got emails from people who are also interested, asking to summarise the results, so here is what I’ve got so far: 1) Liquidator 96 200mkl from Rainin. Everyone agrees it works well and is easy to use, but tips from Rainin are required. 2) Integra VIAFLO 96. Ti

Re: [ccp4bb] Bug in latest COOT

Same with me, I have Mac OS 10.11.6 (El CApitan). I couldn’t add terminal residues, then went three CCP4 updates back, it got OK, but I still can’t add OXT. Olga > On 14 Dec 2016, at 04:51, Jobichen Chacko wrote: > > Dear All, > Any solution to this problem. > I am also encountering this iss

Re: [ccp4bb] crystallization optimization

MMS (microseed matrix screening) into several screens, as Patrick suggested earlier, would be the first choice for me. Works very often. Good luck! Olga > On 12 Jul 2017, at 08:48, Frank von Delft wrote: > > The point I was failing to make: reducing either protein or precipitant > concentr

Re: [ccp4bb] suggestion of crystallization optimization

MMS often works well for us too! Best wishes, Olga > On 5 Jun 2018, at 19:24, Artem Evdokimov wrote: > > Janet, Patrick, and others beat me to it :) > > We have tremendous luck with MMS in cases like this. > > I would also suggest looking at 'atypical' additives - the word atypical is > re

Re: [ccp4bb] To Trim or Not to To Trim

Hi everyone, I always thought it is better to truncate so that biologists looking at the structures are not misled? Not sure it the best aproach though... Olga > On 10 Mar 2023, at 02:45, Bernhard Lechtenberg > <968307750321-dmarc-requ...@jiscmail.ac.uk> wrote: > > Hi Rhys, > > I am a

[ccp4bb] Crystal imagers?

Dear All, Does anyone use Jansci PS256 imager? And if yes, what do you think about it? Is it reliable, easy to use, or has some problems? We are thinking of buying it to replace our Rigaku Minstrel, which has been out of action for more than a year... Thanks a lot! Olga Dr Olga Moroz York