[ccp4bb] off topic - Pharos vs. Typhoon imagers

Hi all, My apologies for the drastically off topic question. Does anyone have experiences, good or bad, that they feel would influence our decision to buy either a Pharos or Typhoon imager? I'm looking for a 3-laser imaging system. Although it will primarily be used for phosphoimaging of

[ccp4bb] Noncrystallography question (human cell line cloning question)

Hi, Sorry for the extremely off topic question. I was wondering if anyone had any opinions on which vector system is best for ectopic expression of a protein of interest in human cells (quiescent or dividing in tissue culture)? This would be for studies of the effect of the protein on the