[ccp4bb] 3D printing format

Sorry for the rather random question but has anyone out there used a 3D printer to print a protein structure? If so, what format did you need to convert the PDB into to allow the printer to interpret the data? Many thanks, Joe P Joe Patel FBLG Specialist _

Re: [ccp4bb] Unusual electron density - any guesses??

Is that a glycine in the sequence next to the Glu/Gln? Have you tried building a 50% occ of the backbone in that region in two conformations, and then a water molecule further up into the feature. The density over the carbonyl looks weak and you have some negative density there that might indi

[ccp4bb] Supplier for X-ray sensitive paper

Hi All, I have done a few searches of the archive and googled a few times but not found what I am looking for. Could someone point me in the direction of a supplier of the X-ray sensitive paper I have used in the past to confirm beam position on a home source. I am specifically after this typ

Re: [ccp4bb] Ligand geometry obs. vs. ideal

Hi Yuri, If you have access to mogul you can get an understanding of what your geometry should be based on the small molecule database. Of course not everything is well represented so if your ligand is unusual this will flag up in lower statistical significance. Mogul will allow you to unders

Re: [ccp4bb] Lithium versus Sodium

Hi Scott I may be completely wrong but I worked on a lithium and sodium inhibited enzyme during my PhD. At the time, it was considered that your chances of actually seeing density for a Li+ ion were slim to nil. Only 2 electrons makes them as tough as hydrogens. My efforts went into trying to

[ccp4bb] Permanent Position for NMR spectroscopist at AstraZeneca, Alderley Park, UK

There is currently a position available within the Structure & Biophysics group. The application deadline is 30th January 2012. Please use the AstraZeneca Careers website to submit your application, if you are interested. The link to the job, with a role description can be found using the link

Re: [ccp4bb] strange density

Hi Alex, Was it purified via a Ni2+ resin? Is the protein oligomeric in solution? Could it have stripped an ion out during purification and brought it all the way through to crystallisation? Have you tried refining a Ni2+ in the location? JP --

Re: [ccp4bb] question about the zinc binding protein

BTW"By the way" I think, not a buffer abbreviation -- AstraZeneca UK Limited is a company incorporated in England and Wales with registered number: 03674842 and a registered office at 15 Stanhope Gate, London W1K 1LN.