Re: [ccp4bb] 60 kDa contamination in Rosetta cells

2021-07-19 Thread PiscoScience
Hvvxbxhxxhhfjxjdjdhhdydfyhat the 60 kDa is not your biggest concern. Optimize soluble protein expression and pay attention to cell lysis. Is the sample remaining cold throughout the process? Are you using enough volume of buffer relative to solid cell pellet? > > Also, from the gel it does not

Re: [ccp4bb] Exciting postdoctoral position in protein complexes operating in human DNA replication by CryoEM in Leicester

2020-09-26 Thread PiscoScience
El El jue, 24 de sep. de 2020 a la(s) 11:48, Videler, Tennie (Dr.) < h...@leicester.ac.uk> escribió: > > > > > > > > > > > > > > > *Apologies for cross-posting*​ > > > > > > > > > > > > > > > > > > Dear all, > > > we are looking for highly > > motivated candidates to apply for a 2-year