Hi Group:
I have a peptide that got crystallized within 20-25 minutes after putting down
the tray.
The condition has 1.8M Ammonium Salt, 0.01 Cobalt (ii) Chloride hexahydrate and
MES (0.1M) pH 6.5.
I dissolved my peptide in 25 mM Sodium Accetate (pH=5).
PI of my peptide is 10.2
When I checked
I think the chain identifier for both the states is same. If it is, change it
and hopefully it should work .
Cheers,
Prasun
From: CCP4 bulletin board on behalf of Sam Tang
Sent: 30 January 2021 13:24
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Saving multi-sta
Hi All:
I am trying to solve a structure that has got D-amino acids. The sequence has
also got few achiral residues but is mentioned as a part of L-peptide in the
monomer library. For example GLY or AIB. Since my sequence has all D-amino
acids, I was thinking of some method by which these achir
Hi Group
I am refining one of the structures that has modified amino acid and it is not
defined in the CCP4 dictionary.
I got the restrains using ELBOW and used it for refinement. However, when I
refine the structure using REFMAC, the bond length deviates significantly from
the ideal/ given val
, Winfried
Sent: 16 November 2021 19:24
To: Prasun Kumar
Subject: Re: [ccp4bb] Refmac is changing the bond lengths, torsion angle of
modified residues
Dear PR,
0.1Angstrom is not a general disaster - you should talk to a chemist.
Best,
Winfried Hinrichs
Hi All:
I have performed racemic crystallography and got crystals that diffracted. The
automatic processing softwares, XIA2 DIALS, (available in Diamond, UK) gives
the space group as P1 and cell dimension
42.78 52.16 54.49 114.11 92.16 92.31.
Challanges start from here:
1) How much
Thank You Pierre!
I will have a look and let you know.
Regards
Prasun
From: LEGRAND Pierre
Sent: 04 March 2019 16:33:56
To: Prasun Kumar; CCP4BB@JISCMAIL.AC.UK
Subject: RE:[ccp4bb] Racemic crystallography and structure solving problem
Hi Prasum,
In such
replacement is a problem for me. However, I am on it.
Regards
Prasun
From: Tim Gruene
Sent: 05 March 2019 10:02:53
To: Prasun Kumar
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] Racemic crystallography and structure solving problem
Dear Prasun,
in case you
Hi All
Happy New Year to All.
I have a PDB file that has left-handed 310 helices. However, the helix class in
PDB documentation
(https://www.wwpdb.org/documentation/file-format-content/format33/sect5.html)
does not have any number assigned to the left-handed 310-helix.
The auto annotation soft
Hi All:
I have collected a dataset for a crystal of a 30 residues long helical peptide
that makes a trimer in the solution. I also solved the structure to get a
trimer. My issues start when I start preparing for a deposition.
Details about the data:
space group: I 21 3
Resolution: 1.6
Current
Hi Group
I currently updated my ccp4 to the latest version. I have also made python3.7
the default version.
The log when I run ccp4i2 & from the command line is appended below. I suspect
it is the python version but please guide me for the same.
Python 3.7.12 (default, Jan 6 2022, 21:30:12)
[
Doc
File "src/lxml/parser.pxi", line 711, in lxml.etree._handleParseResult
File "src/lxml/parser.pxi", line 640, in lxml.etree._raiseParseError
File "", line 1
lxml.etree.XMLSyntaxError: Document is empty, line 1, column 1
From: Bo
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