[ccp4bb] Summary: Ordering a cDNA library

: Rajan, have a look at http://www.imagenes-bio.de. I guess there are more suppliers. Please send a summary to the board. Good luck, Guenter Rajan Pillai wrote: Hi All, Apologies for a non-CCP4 question. I want to clone a couple of proteins of human and mouse origin. Can anyone tell me

[ccp4bb] Review on Membrane Protein Purification and Crystallization

Hi All, Is there a good review or special issue of a journal dedicated to the problems associated with membrane protein purification and crystallization? Key points of focus would be on overcoming the solubility problem, stability and techniques of crystallization. Thanks, Rajan

[ccp4bb] Ordering a cDNA library

Hi All, Apologies for a non-CCP4 question. I want to clone a couple of proteins of human and mouse origin. Can anyone tell me where from I can order Human and mouse (brain/liver) cDNA library? Any suggestions are welcome. Thanks, Rajan

Re: [ccp4bb] Clarification of MOLREP logfile

Hi All, In the portion of the MOREP logfile where it lists the RF peaks, what does Rf and Rf/sigma mean? Is Rf - peak height and Rf/sigma - peak heights measured as deviations from mean? Looking into the CCP4 tutorial on MOLREP (http://www.ccp4.ac.uk/dist/examples/tutorial/html/mr-tutorial.html)

[ccp4bb] Clarification of MOLREP logfile

Hi All, In the portion of the MOREP logfile where it lists the RF peaks, what does Rf and Rf/sigma mean? Is Rf - peak height and Rf/sigma - peak heights measured as deviations from mean? Looking into the CCP4 tutorial on MOLREP (http://www.ccp4.ac.uk/dist/examples/tutorial/html/mr-tutorial.html)

[ccp4bb] Rotation Function of MOLREP

Hi All, I want to plot the rotation function that MOLREP uses. I cannot find any output of rotation function in the logfile or in moIrep.doc. I want to locate the peaks of the rotation function, that are shortlisted as solutions, which would help me in understanding the following problem. My

[ccp4bb] Resolution limit for Molecular Replacement

Hi All, I read some literature for phasing by molecular replacement performed with reflections upto 3.5 Angstroms. Can anybody tell me why? I would prefer deleting low resolutions so as to reduce contribution from the solvent that might affect RF search and obtaining a solution. Your responses to

Re: [ccp4bb] Ratio of Number of Reflections to Number of restrained Parameters

The question was well crafted. It behooves on part of the respondent to understand the question or clarify before answering. On Fri, Aug 29, 2008 at 2:24 PM, Ian Tickle [EMAIL PROTECTED] wrote: Agreed - but it wasn't entirely clear from the original question that this was the purpose of the

[ccp4bb] Ratio of Number of Reflections to Number of restrained Parameters

Hi All, I was wondering if there is any program that calculates the ratio of number of reflections (observation) to number of restrained parameters. If not then how it can be calculated. The number of reflections is it number of unique reflections or number of the total reflections? Thanks,

[ccp4bb] Ligand Binding

Hi All, I want to confirm the binding of the substrate in the active site of the enzyme by soaking crystals of the apo-protein with the substrate. I generated a Fobs_lig - Fobs_apo difference electron density map. Inspection of the map did not show the characteristic electron density of the

[ccp4bb] Phased Anomalous Difference Map

Hi All, Can anyone tell me how I calculate a phased anomalous difference map? I want to confirm such as the presence of Cl or S ion in an unknown electron density. Rajan.

[ccp4bb] Calculating volume of Ligands

Hi All, Can anyone tell me any program that calculates voume of a ligand? Moreover, is there also any program that can calculate the volume of a ligand from its coordinates? Thanks, Rajan.

[ccp4bb] Measuring the volume and surface area of an active site

Hi All, Can anyone tell me if there is any program available that can measure the surface area and volume of the active site of a protein? Thanks, Rajan.