Re: [ccp4bb] Codon Optimization for Insect Expression

2013-05-09 Thread Roger Pickman
I hope this isn't too much of a thread hijack, but i also wanted to add a question to Randy's : How big a protein can one express in insect cells? I've read in a few places that it's not size limited but i wonder if that's borne out in practice. On 9 May 2013 16:05, Randy Watson

[ccp4bb] Binding constants/kinetics for crystallisation

2012-12-07 Thread Roger Pickman
Dear all - is there a rule of thumb for favourable values of Kd, kon and koff of protein-protein or protein-dna complexes for protein crystallisation? Are these measurements useful in crystallisation, or should one just put it down a gel filtration column, hope for a complex and not worry? If