[ccp4bb] Superposition of maps in diferent spacegroups

2008-07-30 Thread Ronaldo Alves Pinto Nagem
Dear CCP4 users, I was using MAPPROT from CCP4 package to rotate/translate an electron density map in order to superpose maps from different space group crystals. However, when applying the same operations (euler angles + translation OR rotation + translation matrices) into the maps and into the

[ccp4bb] Summary: Superposition of maps in diferent spacegroups

2008-07-30 Thread Ronaldo Alves Pinto Nagem
Summary: Problem: I was using MAPPROT from CCP4 package to rotate/translate an electron density map in order to superpose maps from different space group crystals. However, when applying the same operations into the maps and into the pdb files the maps and the pdb files do not superpose. I tried

[ccp4bb] Unidentified ligand (electron density) found at active site

2007-11-30 Thread Ronaldo Alves Pinto Nagem
Dear crystallographers, I hope someone could give me a hint to solve this problem. I have collected five datasets from the same protein and all of them, including the highest resolution data set (1.6 angstrons), display at the active site of the enzyme a very clear electron density with

[ccp4bb] Main topic of the day: Protein crystallization

2007-02-22 Thread Ronaldo Alves Pinto Nagem
Dear all, As protein crystallization is the main topic of the day may I include another question? What was the minimun protein concentration reported with success in crystallization trials? I ask that because the protein I am trying to crystallize is much less soluble than the one mentioned in