Re: [ccp4bb] Problems with refining covalently linked heme cofactor

Try Buster! Pietro Get Outlook for iOS From: CCP4 bulletin board on behalf of Vera Pfanzagl Sent: Tuesday, July 13, 2021 3:33:04 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Problems with refining covalently linked heme cofactor Hello

Re: [ccp4bb] all of a sudden I cannot launch Coot nor ccp4i from the Terminal on my Mac laptop

Skype: roversipietro Mobile phone +44 (0) 7927952047 Tel. +44 (0)116 2297237 From: CCP4 bulletin board on behalf of Roversi, Pietro (Dr.) Sent: 09 March 2021 17:04 To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] all of a sudden I cannot launch Coot nor ccp4i fr

[ccp4bb] all of a sudden I cannot launch Coot nor ccp4i from the Terminal on my Mac laptop

Dear all, sorry to bother you all with such mundane headaches - but all of a sudden I cannot launch Coot nor ccp4i from the Terminal on my Mac laptop (running MacOS Mojave): [bioch3242-596:~] pietro% coot(coot-bin:15697): GLib-GObject-WARNING **: g_object_set_valist: object class 'GtkSettings'

[ccp4bb] Depositing structures of proteins of unknown sequence

Dear all, I am fitting a 25 Å negative stain EM map of a protein in complex with its FAb. I have restraints on the protein region the FAb recognises (from ELISAs on truncated versions of the protein) but I do not have the sequence of the FAb. I have a model for the protein and I picked a FAb fr

Re: [ccp4bb] What refinement programs are fully Open Source?

ell Biology, University of Leicester Henry Wellcome Building Lancaster Road, Leicester, LE1 7HB England, United Kingdom Skype: roversipietro Mobile phone +44 (0) 7927952047 Tel. +44 (0)116 2297237 From: Ethan A Merritt Sent: 07 May 2020 18:08 To: Roversi

[ccp4bb] What refinement programs are fully Open Source?

Dear all, we are in the editorial stages of a manuscript that I submitted to Wellcome Open Research for publication. The journal/editor ask us to list fully Open Source alternatives to the pieces of software we used, for example for data processing and refinement. What refinement programs are

Re: [ccp4bb] Expi 293 (Met-) expression medium for selenomethionine labeling

Dear Laura, a few years ago - when I realised that mamalian cell expression media *without Met* costed much more than *with Met* because it is classified as custom media - I tried to persuade the rep that there was a market for it - to no avail. I did then plan an experiment in which I would de

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

Dear all, my twopenny worth - when Helen Walden and I put together the 2013 CCP4 Weekend list of speakers, we were delighted to end up with a not-so-gender-imbalanced list of speakers, but we did achieve that balance *intentionally*. As we attempt to redress a de facto longstanding bias against

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Cell Biology, University of Leicester Henry Wellcome Building Lancaster Road, Leicester, LE1 7HB England, United Kingdom Tel. +44 (0)116 2297237 From: DUMAS Philippe (IGBMC) Sent: 05 March 2019 11:20 To: Roversi, Pietro (Dr.) Cc: CCP4BB@JISCMAIL.AC.UK Subject: Re

[ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Dear all, I'd like to purchase slides with diffraction gratings for educational purposes. Ideally, to be used with a visible-light laser pointer. And: with 1D and 2D patterns, of variable repeats - so as to be able to illustrate the effect on the spacing in reciprocal space, as it were. I am l