Re: [ccp4bb] Crystallizing protein sitting in PBS

Of course PBS should not be a first choice for screning crystal. But I will try all kinds of buffer until I got the structure. Nobady can tell you that you can't get crystal in the PBS buffer. and I will shot everything I have if I have enough beam time. Good luck.

Re: [ccp4bb] co-crystallization

for me, I prefer to sock these compounds into your crystal. it will much more easy than co-crystallizaiton. But each protein should be different. Normally when I star to co-crystallization with small compound, I will set up the complex with 1:1.2 molar ratio as first trial to see what should hap

Re: [ccp4bb] structure based superposition

normaly you can do it in the pymol. In the main interface, on the left menu, there is a:"A" button, click it, then looking for "Aliagn". Then you can do it. But I prefer to use CCP4, in CCp4 there is a tool : Superpose. You can fine any region you want to do superpose.

Re: [ccp4bb] reproducibility of protein crystals

sometimes， a little bit change of concentration of your precipitate or pH buffer can affect your crystal. My suggestion is setup a appropriate gradient of your precipitate and additive.