Mono-S column to separate the Fab
isoforms from (Fab)2 and undigested IgG. The purified Fab mixed with antigen
1:1.2 molar ratio and used the gel filtration to separate the complex from
unbound antigen.
Hope this will help you
Cheers
Sivaraman Padavattan
On Fri, Sep 10, 2010 at 8:29 AM
Dear all,
I am performing an assay to screen for the inhibitor molecules. One of our
compound shows inhibition at 100 μM and activation at 500μM conc. What could
be the possible reason for the compound work differently in two different
conc.
Thanks
Sivaraman
?
Thanks in advance,
Sivaraman Padavattan
protein
free of nucleic acid contamination.
Thanks in advance,
Sivaraman Padavattan
s and Cons) on the above
machines, which would help us in the decision process.
Greatly appreciate your response. Thank you for your valuable time.
Best,
Sivaraman Padavattan
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