Re: [ccp4bb] Lost my protein

Hi everyone, It seems that our lab has experienced issues with precipitation of some proteins (not all) with the Amicon concentrators. For those, we have started using VivaSpin concentrators. That seems to work fairly well with those that have issues with the filters. You may want to give it a try.

Re: [ccp4bb] The Lawnmower Man

For the Apple fans out there, there's some rumors that they may be developing 3D display hardware without the need for glasses: http://www.appleinsider.com/articles/08/03/20/apple_researching_autostereoscopic_3_d_display_hardware.html As for the gaming glasses from the original question, there's

Re: [ccp4bb] multiple sequence alignment from multiple pairwise structural alignments

Hi Stephen, Fun question! A quick google search with "structure based sequence alignment" gave me this site which looks promising: http://www.cgl.ucsf.edu/home/meng/grpmt/structalign-content.html Let me know how it turns out! Sophia On Tue, Mar 4, 2008 at 11:13 AM, Stephen Graham <[EMAIL PROTEC

Re: [ccp4bb] crashing-out protein eluted from Nickel column

Hi, Agreed on this. I used to have issues with aggregation due to the Nickel being stripped off the column (happens with elution in imidazole). Adding 10mM EDTA to the elution immediately AFTER it has come out of the column will chelate the Ni++ and prevents aggregation (at least in my case). Afte