Re: [ccp4bb] Protein melting temperatures

2010-09-23 Thread Thomas J Magliery PhD
I agree that you can't take two unrelated proteins and expect their Thermofluor Tms will be correlated with CD/DSC values. We've done quite a bit with point mutants, and it works well for that (see an example in our paper below). Also note that the dye is a perturbant the reduces the

[ccp4bb] updating refmac

2009-11-16 Thread Thomas J Magliery PhD
I wanted to update refmac5 to the latest version in CCP4. I downloaded v5.5.0105 and unpacked the tarball. The three files are named refmacgfortran, libcheckgfortran and makecifgfortran. So, I moved the corresponding files refmac5, libcheck and makecif in ccp4-6.1.2/bin/ to another folder

[ccp4bb] [Fwd: [ccp4bb] CCP4 / Ubuntu 9.04 64-bit]--Coot

2009-07-06 Thread Thomas J Magliery PhD
/showthread.php?t=474790 http://www.ccp4.ac.uk/martyn/ubuntu.html 3. I also never tried the 64-bit CentOS binaries, but that might be worth a try. Tom Original Message Subject:[ccp4bb] CCP4 / Ubuntu 9.04 64-bit Date: Fri, 3 Jul 2009 17:18:27 -0400 From: Thomas J Magliery PhD

[ccp4bb] CCP4 / Ubuntu 9.04 64-bit

2009-07-03 Thread Thomas J Magliery PhD
Hi, I just installed the 64-bit version of Ubuntu 9.04 Desktop, and then I installed CCP4 6.1.1 using the standard package and installation script. I can run ccp4i successfully. However, when I try to run Coot, I get a bunch of errors. I think the problem is that Coot was compiled with

[ccp4bb] Refmac 5.4 monomer dictionary mirror?

2008-05-19 Thread Thomas J Magliery PhD
I am trying to upgrade Refmac from 5.2 to 5.4. I didn't download the monomer library (refmac5.4_dictonary.tar.gz) when I downloaded the executables, and I can't access the Refmac web page today. Is that file mirrored somewhere, or can someone provide it to me? Thanks. Tom -- Thomas J.

Re: [ccp4bb] how to change a membrane protein into a water soluble protein?

2007-12-03 Thread Thomas J Magliery PhD
It's hard. See: J Mol Biol. 2005 May 6;348(3):777-87. X-ray structure of a water-soluble analog of the membrane protein phospholamban: sequence determinants defining the topology of tetrameric and pentameric coiled coils. Slovic AM, Stayrook SE, North B, Degrado WF. Slovic, A. M., Summa, C.