Re: [ccp4bb] Crystallization of low solubility proteins from glycerol-containing solutions

2010-08-25 Thread Tom Walter
there is a big variation in price dependent on which particular one you use. Use them at ~200-300 mM and you may even be able to get rid of the glycerol. You may have to incubate the protein with the NDSBs overnight before concentration to get the full effect. Good luck Tom Walter Original message

Re: [ccp4bb] problem of crystallization

2008-05-14 Thread Tom Walter
** Tom Walter B.Sc. M.Res. ** ** Oxford Protein Production FacilityTel: +44 (0)1865 287747 ** ** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547 ** ** Roosevelt Drive [EMAIL PROTECTED] ** ** Headington, Oxford

Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

2008-04-15 Thread Tom Walter
...) we have two machines (and another one imminent) to increase throughput. HTH Tom ** Tom Walter B.Sc. M.Res. ** ** Oxford Protein Production FacilityTel: +44 (0)1865 287747 ** ** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547

Re: [ccp4bb] which concentrated salt has lowest vapour pressure?

2008-04-09 Thread Tom Walter
that helps Tom ** Tom Walter B.Sc. M.Res. ** ** Oxford Protein Production FacilityTel: +44 (0)1865 287747 ** ** Wellcome Trust Centre for Human Genetics Fax: +44 (0)1865 287547 ** ** Roosevelt Drive [EMAIL PROTECTED

Re: [ccp4bb] crystallization robot

2008-01-09 Thread Tom Walter
and disadvantages so it just depends what you will be using them for, what disadvantages you are willing to put up with, and of course your budget. Happy shopping! Tom ** Tom Walter B.Sc. M.Res. ** ** Oxford Protein Production FacilityTel: +44 (0)1865 287747