Re: [ccp4bb] real space refinement broken in COOT 0.9.8.8 EL?

2023-07-17 Thread Tsybovsky, Yaroslav (NIH/NCI) [C]
Hello, I can confirm that this happens in 0.9.8.7. The problem is intermittent and I have not been able to determine what causes it so far. Some residues just fall apart into atoms as a result of real space refinement. Best, Yaroslav Yaroslav Tsybovsky, Ph.D. (contractor) Senior Scientist & He

[ccp4bb] Zero defocus range after CTF refinement

2023-02-23 Thread Tsybovsky, Yaroslav (NIH/NCI) [C]
Dear community, Occasionally I deal with datasets for which CTF refinement in Relion (both 3 and 4) makes essentially no changes in defocus for any particle in any movie (example graph attached). Does this mean the data is not "strong" enough for CTF refinement or are there any know tricks to m