Re: [ccp4bb] Cobra Cryodrive 2.0 from Oxford Cryosystems to give away

The Cryodrive is already gone. Best, Ulrike To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of

Re: [ccp4bb] glass capillaries

The capillaries have already found a new owner ! Ulrike To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB=1 This message was issued to members of

[ccp4bb] glass capillaries

Dear all, is anyone still using glass capillaries for crstal mounting at room temperature ??? We have a couple of them in size from 0.2 - 2.0 mm diameter and 80 mm length. I would be happy to give them away. Best, Ulrike

Re: [ccp4bb] honeybee repair?

Hi Jon, here in Germany we got the Honey Bee installed by Zinsser Analytic. May be they are still doing the service . http://www.medicalexpo.de/prod/zinsser-analytic/product-70627-771591.html You have to scroll down the page a bit to find Zinsser. Good luck ! Ulrike

Re: [ccp4bb] cryo protection for low salt crystallization at 4 degrees

Hi Ursula, you could try Perfluoropolyether PFO-X175/08 (Hampton Research HR2-814). You just plunge your crystals in the liquid or wipe the crystal in the loop through the liquid so that all excess water is removed. Good luck ! Ulrike

[ccp4bb] Skin on drops

Dear crystallographers, I am trying to crystallize a soluble protein which tends to form aggregates. The crystallization condition is 20% PEG 3350 + 0,2 M Na-Formate. During the crstallization process a thick skin is formed on top of the sitting-drops. As well the crystals are buried in

[ccp4bb] Incubator for crystallization

Hi everybody, I want to buy a smaller incubator for protein crystallization (30-50 l). Do you have any recommendations ? Do you prefer specialized incubator models with low vibration or are you just using standard incubators ? Best wishes, Ulrike

Re: [ccp4bb] salt or not?

Dear Careina, altough your crystals does't take up the Izit dye it sounds promising. The uptake of Izit depends on the solvent channels of the protein molecule - sometimes the dye just can't enter the molecule. Concerning the Calciumchloride - if the concentration is not too high and without

Re: [ccp4bb] shipping dewar case

Dear Mike, we got a dry shipper from Taylor Wharton ( http://www.taylorwharton.com/default.aspx?pageid=72). In Europe dry shippers and accessories are sold by Molecular Dimensions (http://www.moleculardimensions.com/shopdisplayproducts.asp?Search=Yessppp=6). Cheers, Ulrike

Re: [ccp4bb] crystal dehydration

Dear Judith, in Acta Cryst D61 (2005), 1173-1180 different handling methods for dehydration are described besides other post-crystallization treatments to improve diffraction quality. You can also try Dehydration Salts. A screen with ready made salt solutions is available from Jena Bioscience.

Re: [ccp4bb] Expert opinion for optimizing ethylene glycol crystallization condition

Dear Shankar, I once got crystals with Ethylene glycol as precipitant but at low concentration (8-14%) and there always had been spots right from the beginning. It sometimes happens that you get nice looking crystals but they do not diffract. If you have hits from other conditions may be you

Re: [ccp4bb] Perfluoropolyether as cryoprotectant for membrane proteins ?

Thank you all for sharing your experience with Perfluoropolyether and freezing membrane proteins ! Now I myself heard about one membrane protein where Perfluoropolyether was the solution for the freezing problem. And it seems it worked in my case as well :-) Ulrike

[ccp4bb] Perfluoropolyether as cryoprotectant for membrane proteins ?

Dear crystallographers, does anyone have experience using Perfluoropolyether (Hampton Research) as cryoprotectant for membrane proteins ? Cheers, Ulrike

Re: [ccp4bb] Problems in crystallization

Dear Eva, are you using siliconized cover slips or normal ones ? Protein drops on not siliconized glass tend to spread especially if they contain alcohol or detergent. But this shouldn't be a problem with sitting drops usually. Good luck ! Ulrike

Re: [ccp4bb] cryo conditions

Dear Ed, try ca. 30 % PEG 400 Another possibility could be Perfluoropolyether Cryo Oil from Hampton Research. This liquid doesn't mix with water. You put the crystal in few microliter and remove the excess water droplets from your screen solution (otherwise you will get ice rings). Cheers,

Re: [ccp4bb] Improvement in crystal quality

Dear Nishant, are you sure your crystals are protein ? The condition contains 1 M of different salts and PEG as well - I wouldn't exclude that you are having salt crystals. You can easy check this with Izit dye or simply by trying to destroy the crystal with a microtool. If it is salt you can

[ccp4bb] Dialysis Buttons

Dear crystallographers, does anyone know if dialysis buttons for crystallization are available somewhere in Europe or only at Hampton Research ? Thanks, Ulrike

[ccp4bb] Butandiol as cryopotectant

Hi everybody, has anyone experience with Butandiol and its different isoforms as cryoprotectant ? Thanks, Ulrike

Re: [ccp4bb] Crystallization Reagent

Dear Paul, AMP-PNP is available from Sigma-Aldrich. Product numbers are A 2647 and 01910. Best wishes, Ulrike