Re: [ccp4bb] Crystallization condition for trimeric protein

2013-09-16 Thread Vandna Kukshal
gnostics > Hyderabad, INDIA > > Email(s): dkgh...@cdfd.org.in, dgho...@gmail.com > Telephone: 0091-9088787619 (M), 0091-40-24749396 (Lab) > Lab URL: > http://www.cdfd.org.in/labpages/computational_functional_genomics.html > -- Vandna Kukshal Postdoctral Research Associate Dept. Bio

Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Vandna Kukshal
Hi , Seems to me that crystal packing is not good thats why mosaic diffraction, this might be due to DNA so you can increase diffraction quality by using 1 or 2 nucleotide sticky end DNA for crystallization which can help in crystal packing . Vandna Kukshal Postdoctral Research Associate

Re: [ccp4bb] Dose anyone see this ligand before?

2013-07-16 Thread Vandna Kukshal
t; any purpose. Thank you for your cooperation. > > > .. > Jürgen Bosch > Johns Hopkins University > Bloomberg School of Public Health > Department of Biochemistry & Molecular Biology > Johns Hopkins Malaria Research Institute > 615 North Wolfe St

Re: [ccp4bb] STRAP secondary structure

2013-04-27 Thread Vandna Kukshal
iated. > > Thanks > Mike > > -- Vandna Kukshal Postdoctral Research Associate Dept. Biochemistry and Molecular Biophysics Washington University School of Medicine 660 S. Euclid, Campus Box 8231 St. Louis, MO 63110

Re: [ccp4bb] unidentified density

2012-10-17 Thread Vandna Kukshal
I think it might be PEG. u should try to fit that. On Wed, Oct 17, 2012 at 7:13 AM, RHYS GRINTER < r.grinte...@research.gla.ac.uk> wrote: > Is this density on an 2 fold axis? > > From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Sudhir > Kuma

Re: [ccp4bb] Nobel Prize 2012

2012-10-10 Thread Vandna Kukshal
many congratulations to protein structure community ... On Wed, Oct 10, 2012 at 6:20 AM, Toufic El Arnaout wrote: > Congratulations to Robert Lefkowitz and Brian Kobilka: > Nobel Prize in Chemistry, 2012! > Congrats to the protein structure community. > > > > toufic el arnaout > --

Re: [ccp4bb] precipitation.

2012-03-01 Thread Vandna Kukshal
hi rashmi ,,, I have one suggestion dont use KCL in your buffer for crystallization b'coz u ll get lots of salt crystals mostly of K2SO4 . i faced this problem for one of my halophilic protein . most of the condition where NH4So4 is there u ll get salt crystals. On Thu, Mar 1,