Re: [ccp4bb] Name that protein!

It is very similar to a voltage-gated potassium or sodium channel. Beautiful structure! Get Outlook for Android From: CCP4 bulletin board on behalf of Sorin Draga Sent: Wednesday, June 27, 2018 1:41:47 PM To: CCP4BB@JISCMAIL.AC.UK Subject

Re: [ccp4bb] Help with assigning density

Hi Tristan! Definitely PEG, in the so called horseshoe shape. Cheers, Mirella Get Outlook for Android From: CCP4 bulletin board on behalf of Tristan Croll Sent: Friday, January 26, 2018 6:09:01 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [c

Re: [ccp4bb] Predict effects of mutations on protein stability and protein-protein interaction

Dear Wenhe, I have used iMutant 2.0. I hope it helps. Below the link of a paper related to the prediction server: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1160136/ Best, Mirella Get Outlook for Android From: CCP4 bulletin board on beh

Re: [ccp4bb] off topic

Hi Anamika, I guess you tried several growth conditions. You might try to do a heat shock growth. Grow at 37C until OD 0.6, then move the bacteria to 42C for half hour, then to 30C and grow them for 20 hours. Another strategy is to add just before induction 1,2 or 3% Ethanol. Not sure these will w

Re: [ccp4bb] Possibility of Quasi-crystal formation of heterodimeric protein complex

Dear Debiasish, it looks like you got spherulites. You might try to optimize the conditions or use these spherulites for microseeding. It worked for me once. Good luck! Mirella Sent from my iPhone On 24 Mar 2017, at 08:29, Camillo Rosano mailto:camillo.ros...@gmail.com>> wrote: Dear Debiasish

Re: [ccp4bb] How to determine the concentration of biotinylated peptide?

Hi Alex, you can measure the absorbance at 214-220 nm, which is where the peptide bonds absorb, but you should know/calculate/predict the extinction coefficient of your peptide at that wavelength. Furthermore, you might try BCA assay which is colorimetric as the bradford but the reaction involv

Re: [ccp4bb] Unknown electron density blob

Hi there, I think it is PEG, very common to have PEG in horseshoe shape with arginine pointing toward to it. Cheers, Mirella Vivoli Mirella Associate Research Fellow Biocatalysis Centre, Henry Wellcome Building College of Life and Environmental Sciences, University of Exeter Stocker Road

Re: [ccp4bb] off-topic: protein losing FAD during purification

Dr. Stefano, 1) I would add FAD in all buffers after lysis,even after GF you could perform dialysis with buffer supplemented with FAD. 2)for the second question I found this paper which could be helpful: Large-scale preparation and reconstitution of apo-flavoproteins with special reference to but

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340 nm). I would try to decrease the concentration of your protein to 4-5 mg/ml.Good luck. Best, Mirella Vivoli Mirella Postdoctoral Fellow University of Exeter, Biosciences Biocatalysis Centre, Henry Wellcome Building Stocker Road, Exeter, Ex4 4QD Tel:+ 44 (0)1392 726

Re: [ccp4bb] problems with cleaving protein

Best, Mirella Vivoli Mirella Postdoctoral Fellow University of Exeter, Biosciences Biocatalysis Centre, Henry Wellcome Building Stocker Road, Exeter, Ex4 4QD Tel:+ 44 (0)1392 726121 From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of K Singh