Re: [ccp4bb] Regarding NACCESS Server for Protein solvent accessible surface

2022-04-22 Thread Wiener, Michael C (mcw2s)
Another golden oldie: GETAREA remains maintained http://curie.utmb.edu/getarea.html Regards, -MW From: CCP4 bulletin board on behalf of Jon Cooper <488a26d62010-dmarc-requ...@jiscmail.ac.uk> Date: Friday, April 22, 2022 at 8:38 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Regarding

Re: [ccp4bb] Strategies for increasing Trp content of proteins

2022-02-19 Thread Wiener, Michael C (mcw2s)
Dear Jack, While we did this for Met (rather than Trp) insertion, (rather long ago) we used multiple sequence alignment to look for orthologs that had Met at positions where no Met was in our target protein. This worked to increase the # of methionines from two to eight, which enabled (rather)

Re: [ccp4bb] Looking for proteins for undergraduate biochemistry lab

2021-06-17 Thread Wiener, Michael C (mcw2s)
Indeed, and its applications (see below) are, umm, quite interesting, both in commercial/industrial food processing and “molecular gastronomy” [great phrase there, eh?] https://modernistpantry.com/products/activa-rm-transglutaminase.html -MW From: CCP4 bulletin board on behalf of Patrick Loll

Re: [ccp4bb] Fluka-branded PEG 3000

2021-03-23 Thread Wiener, Michael C (mcw2s)
During a prior period of great concern about variable peroxidation levels of commercial PEGs (and of polyoxyethylene detergents), we measured/monitored peroxide levels of solutions. The Pierce kit is now available from Fisher https://www.fishersci.com/shop/products/pierce-quantitative-peroxide-a