Re: [ccp4bb] Two Equally Good MR Solutions Found by Phaser

to set the occupancy of any differences in the solution to 0.00 and check from the maps after refinement if you can see which copy of the molecule fits the difference density best - it would nice if you had a TRP/ALA pair of residues or something very distinctive.. Eleanor X Xiong, Cellular

[ccp4bb] Two Equally Good MR Solutions Found by Phaser

Dear Crystallographers, We got a highly repetitive dimeric protein solved by SeMet-SAD in P21 crystal form, and I am now trying to solve a dataset collected from a non-reproducible orthorhombic crystal of the same protein using the structure refined from P21 data. From the Scala statistics,

Re: [ccp4bb] Two Equally Good MR Solutions Found by Phaser

Hi, Thanks for all the replies. Paul said the two solutions are the same by crystallographical symmetry, does that mean the origin of the unit cell has changed and P21212 can have arbitrary origin of the cell along the b-axis? As I have generated the symmetry related pairs from each solution

[ccp4bb] How to Model Methylated N-terminal Amine in Coot

Dear All, My protein has been reductively methylated, and experimental density shows that lysines and N-terminal amines are methylated, while its is easy to model methylated lysine in coot, I can hardly find a way to model the methylated N-terminal. Does anyone know how to do this? Will the