Re: [ccp4bb] stoichiometry of complex and incubator

Hi Min, Given the limited supply of your protein you could try the EDC-NHS zero-length crosslinking either in one-step or two-step protocol (Anal. Biochem. 185,131,1990). If your protein complex is like most protein complexes chances are there will be some electrostatic contacts at the

[ccp4bb]

SET CCP4BB REPRO

[ccp4bb] X-ray equipment available

. Probably good for parts. The equipment is located in Watertown Massachusetts. If interested you will have to arrange for the transport. For more information please contact me directly. With kind regards Zenon Grabarek Principal Scientist Boston Biomedical Research Institute Watertown MA 02472, USA