Dear Members,How well is extinction coefficient based protein concentration
measurement accuracy established. It seems that several monograph based protein
HPLC assays use 214 for the same. How accurate are extinction coefficients for
the same proteins. Is there any consensus if which one is more accurate for
estimation. Some glycoprotein monographs use extinction coefficients.
Thanks ahead Anita Ramdas Patil, Ph.D.Group Leader, ADLWockhardt Research
Center,Aurangabad,INDIA Email: patilramdasanita@yahoo.comMobile# 91+8980217260
