hi Min, I think SPR(Surface plasmon resonance) should suit your situation. The Biacore instrument I worked on required 200 µL of 10-50 µG protein(ligate) for immobilisation and 150µL of various concentration of the other protein(ligand) in the range of 50-1000 µg/ml. (depending on your situation the concentrations may vary)
I hope you can use this technique. Best wishes, Isha Structural Biology Lab, SLS, JNU New Delhi On Thu, Aug 4, 2011 at 8:02 AM, m zhang <mzhang...@hotmail.com> wrote: > Dear all, > > I have two unrelated questions. Any suggestion on any of them will be > greatly appreciated. > > First, we have two proteins that bind each other without a doubt. But since > we have very limited amount of proteins and it takes a long time to > reproduce them, we are very hesitating to try ITC and AUC to find out the > stoichiometry of these complex. So I am wondering if there is any other way > we can try to find the ratio without consuming much proteins? > > Second, we are thinking about getting a new incubator for crystallization. > Could anyone here recommend a model or a brand? > > Thank you, > > Min > >
