[ccp4bb] Off topic_protein degradation.

Dear all, I am trying to crystallize a protein for which the yield and solubility were both fine. However, this protein has a severe problem of degradation. When stored at RT, the protein will degrade madly into pieces, while stored at 4 degree, the degradation is much slower and a relatively

[ccp4bb] bioreactors and fermentors

Dear all, We are planing to buy a bioreactor (fermentors) for insect and mammalian cell culture in our lab, current candidates include WAVE Bioreactor @ GE CelliGen BLU @ New Brunswick BIOSTAT CultiBag RM @ sartorius Our major concern is the size, maintenance easiness, and future consumabl

Re: [ccp4bb] methods to capture proteins from cell culture medium

c. in the sf900 or Hi5 medium is high enough (the formulation is secret, LOL), thus it is good to know that someone has succesful experience with HIC. Thank you very much again! Bei 2011-04-12 joybeiyang 发件人： mi...@chem.ucla.edu 发送时间： 2011-04-12 18:34:27 收件人： joybeiyang 抄送： C

[ccp4bb] methods to capture proteins from cell culture medium

-04-12 joybeiyang

[ccp4bb] off topic-aggregation of proteins

suggestions or comments about how to improve the quality of the protein. Your suggestions and comments will help me a lot and will be highly appreciated. Many thanks to all of you! Bei 2010-09-07 joybeiyang

Re: [ccp4bb] R-Rfree vs resolution

Hi All, Many thanks for replying to my request on "R-Rfree vs resolution". Your wonderful advices are very helpful. 2010-08-05 joybeiyang

[ccp4bb] R-Rfree vs resolution

Dear all, I remember that there used to be a post about the R-Rfree statistics vs different resolution, but can not find it now, could anybody shed a light on me? Thank you very much in advance! Best, Bei 2010-08-03 joybeiyang

[ccp4bb] Summary of : How to distinguish microcrystalline, quasicystalline and precipitation?

pens work, is there any literature to resort to?) Many thanks again to all of you for your nice help! joy 发件人： Emmanuel Saridakis 发送时间： 2010-06-10 17:52:59 收件人： joybeiyang; CCP4BB@JISCMAIL.AC.UK 抄送： 主题： Re: [ccp4bb] How to distinguish microcrystalline, quasicystalline and precipitation

[ccp4bb] How to distinguish microcrystalline, quasicystalline and precipitation?

Hi everyone, I am preparing a "crystallization manual" for our group, however, I found that it is very difficult to distinguish microcrystalline, quasicrystalline and precipitation, especially when the precipitation was shiny, like the grit on the beach. Is there a way to distinguish the three?