Hello, I am sorry to put this question which didn¹t related to the CCP4 software. I couldn¹t get the crystals from the protein only. So I want to try the different way to work it out. I hear that it is possible to set a crystal tray of protein with Trypsin protease. I will be very appreciated about who will provide some detail of this method about how to do this., e.g. How much trypsin shall I add into the protein, How long shall I mix protease with the proteins before I set the tray. Thanks for all. I will also put on the summary of all the answers.
Another question is about protein express in insect cell. 10% of my protein was degradated after 1st purification. Is anyway that I can prohibit this degradation? Thanks Jing
