[ccp4bb] How to slow down crystallization? Need hep!

Hello everyone, I need your suggestion for slowing down crystallization for my proteinmy protein got hit in PEG/ION #5 ( 0.2 M MgCl2, 20% PEG 3350, pH 5.9), but it crystallize too fast. In 1 hr I can see tons of tiny needles. Can anyone give me some suggestion on how to slow down the process? I

[ccp4bb] Need suggestions for protein: ligand ratio for co-crystallization

of excessive peptide in the solution? someone suggested I use lot of peptide , say 3 times of the peptide to protein. usually what is the ratio you will try when you deal with this not-so-small ligand ? any suggestion is appreciated. Lei Feng

[ccp4bb] Free Electron Laser in Stanford?

Sorry to bother those not interested. could anyone share some information on the Free Electron Laser used for crystallography in Stanford? I just heard from friend that they use this extreme high intensity beam to shoot micro crystal. anyone know those , could you please share some

[ccp4bb] please recommend a crystallization incubator

hello everyone can anyone recommend an affordable low temp. incubator that could be used for crystallography? we do not have cold room, so hope the incubator can go to 4 degree withouth too much vibration any size from 10 cubic ft --20 cubic ft will be fine any help is highly

[ccp4bb] Please recommend a microscope for crystallography

hello everyone I really appreciate it if anyone can recommend a microscope for crystallography. I am looking at manual one ( without any fancy motor driven stage or fancy digital camera-computer stuff) , but need have the trinocular for a simple digital camera to take photo I was using

[ccp4bb] 3D system set up

hello everyone I am about to setup a 3D system for crystallography. right now I can only think of coot and pymol software. so I might just go with Windows sytem, plus the Nvidia 3D vision kit I know there is a detailed list of hardware recommended. but are they compatiblility issues?

[ccp4bb] how to make covalent bond between ligand and protein residue in coot?

hello everyone I am wondering how to make covalent bond in coot, between a modified substrate and regular protien residue I can not figure out how to make the bond. Your suggestion is appreciated. Lei

Re: [ccp4bb] Coot 0.6 Released

Hello Paul I am wondering , do we need uninstall the previous version of coot to install this? thanks _ Windows Live Hotmail is faster and more secure than ever.

[ccp4bb] experience with the new coot 0.5.2 and a couple of questions

I just downloaded the new CCP4 package and installed it. comparing with the old version ( I used CCP4 6.0.2 on my windows xp computer and finished my Ph.D projects.), I found the new version of coot is kind of slow in moving to the next residue of the structure by hitting the space. Can

[ccp4bb] protein crystal decay problem

hello everyone, I am seeking help on a crystal decay problem I got crystals of a protein , but it decayed very fast in syncrotron beam. From the initial index, it is P222 space group, I need about 180 images, its initial diffraction went to about 3 A, and after 70-80 images, it droped to