Re: [ccp4bb] To Trim or Not to To Trim

Dear all, to trim or not to to trim…. - what is the difference between highly disordered side-chain atoms versus disordered bulk-solvent atoms? Do not both represent a continuum function with a comparable electron-density distribution? Have not solvent and side-chain atoms merged/mixed at

Re: [ccp4bb] Rational mutations and crystallizability

R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105 Ratzeburger Allee 160 23562 Lübeck Germany -- > Am 08.03.2023 um 13:42 schrieb mesters@biochem > : > > > https://servic

Re: [ccp4bb] Rational mutations and crystallizability

https://services.mbi.ucla.edu/SER/intro.php Best J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Institute of Biochemistry https://www.biochem.uni-luebeck.de phone: +49-451-3101-3105

Re: [ccp4bb] NMR structires

Maybe intensional to stress the word „paradoxal“ included in the title of the deposition? Same could be true for the related entry 1HIS to stress the word „dynamics“? I guess back in in 1993-1994, structures were not checked rigorously… Jeroen -- Dr. math. et dis. nat. Jeroen R. Mesters

Re: [ccp4bb] complementarity between the protein and the ligand/compound

Could this be what you are looking for? http://biosig.unimelb.edu.au/csm_lig/prediction J. -- Dr. math. et dis. nat. Jeroen R. Mesters https://orcid.org/-0001-8532-6699 University of Lübeck Germany -- > Am 12.02.2023 um 03:34 schrieb Xin

Re: [ccp4bb] 3d stereo on windows 10/11

Active stereo support is slowly dying and the hardware will fail in the end (sorry to say)…. One possible alternative is a passive stereo PluraView , which is expensive but compatible with software such as YASARA,

Re: [ccp4bb] Ice Lamellar formation over the MicroTube - Oxford cryo stream 700

Hi, As far as the nitrogen stream delivery system is concerned - remove the leg(s) from the empty dewar and let it warm up to room temperature - make sure the dewar has not collected ice at the bottom. If it has, remove the ice and let the dewar dry - connect a high-vacuum turbo-pump to the leg

Re: [ccp4bb] Regarding the diffraction image

A and B unit cell dimensions are hardly bigger than twice the uni cell of cubic NaCl and will probably not accommodate a 30 kDa protein. Make a SDS gel of washed & dissolved crystals to be sure these are not alt or inhibitor crystals. You can stain the crystals with IzIt... J. -- Dr. math. et