[ccp4bb] precipitation of protein in elution buffer

Hello all I apolozize for a off topic question on BB. I am working with small proetin-protein complex, each have molecular weight 10kDa. I elute this N-terminal His-tagged complex through Ni-NTA resin in 50mM of NaH2Po4, 0.3M of NaCl, 5% glycerol and 250mM of Imidazole.Similarly, Lysis buffer

[ccp4bb] RMSD value is different from O and superpose

Hello all I superposed the two different chain, which are two different subunits of the same structure which are different in conformation with two differen t programmes. 1) First is with O, with LSQ command, it gives the RMSD values for different chain is 1.5A for 108 ca atoms. 2) Second

[ccp4bb] very high concentration of protein

Hello all I am working with a small protein-protein complex. This complex express quite well . I purify in a buffer of pH=9.0 with 150mM NaCl and 1% of glycerol and able to concentrate upto 20 mg per ml. I have a two clones of this protein complex. One is N-terminal His tagged and another

[ccp4bb] precipitation of the protein in crystallisation solution

Hello all I am working on small protein-protein complex of 10 kDa each component.Among these complex one of the partner known to be highly flexible but in complex they are suppose to be well structured and from a tight complex . I am trying to crystallise this complex. When i add the protein in

[ccp4bb] Calculation of angle between two helix of different subunits

Hello all I am interested to know about any programme which can calculate the angle between the helices of different subunit not the consecutive helices. I know about helixang which is a ccp4 supported programme but, i am not sure that does it calculate the relative angle between the two helix of

[ccp4bb] Rotation angle and translation distance

Hi all I am working with a multidomain protein whcih binds to a ligand. This protein exist in two different states, a) apo form of the protein is closed state and b) holo protein is an open state. One of the domain is mobile and have different conformation compared to its closed conformation.

[ccp4bb] Domain rotation axis reprentation

Hi all Sorry for partially off topic query I am working with a multidomain protein. which is present in closed and open conforamtion because of the motion in the domains of the protein. I would like to describe the roation angle of the different domains of the protein and the direction of

[ccp4bb] ligand refinemnet

Hello all I have refined and built model a dataset of 3.0A resolution dataset. This model is assocaied with a ligand. After final refinement and model building i found a big blob of Fo-Fc density of around 4sigma level at the N-terminal of the fianl model. My protein doesnot carry any tag at

[ccp4bb] curvature of a helix

Hello all I would like to calculate the curvature of a curved helix. Is any method or program exists, which can calculate the cuvature of a curved helix? I would appreciate the suggestions. Thanks in advance

[ccp4bb] Angle between two different helices from two different subunits

Hi all I would be interested to know about the programmes which can calculate the angle between the two helices. I want to calculate the angle between the two different helices from two different subunits of the structure. I know a non-supported programme at ccp4 called helixang. Is this