Hi Anamika,
Just to be clear, the M13 ori is for production of ssDNA using a helper phage.
Replication of that plasmid is more likely from another ColE1-derived origin
elsewhere on the vector and also likely to give high copy numbers - which it
seems it does. The p15 ori is indeed quite a low c
Here is another one from a few years ago. Monomeric in solution. Turned out
that the cys in question is a redox sensor in the yeast nucleus (which we
didn’t know at the time) so don’t necessarily dismiss these as xtal artefacts!
Lesson learnt here 😊
http://www.rcsb.org/structure/1SW6
Steve Sme
