Dear all,
Thank you very much for all your helpful comments. I will try them and post
on the BB my results.
Best regards
Shankar
On Wed, Jan 2, 2013 at 11:59 AM, Sankaranarayanan Srinivasan <
texs...@gmail.com> wrote:
> Dear all,
>
> A very happy new year to all.
>
> I
Dear all,
A very happy new year to all.
I would appreciate some expert advice on optimizing a crystallization
condition in which the initial hits were obtained with ethylene glycol as
the main precipitant. Here is the summary of things tried.
We have a protein, size (31Kda) and the starting prot
This paper on thermofluor is a good reference and if you have access to a
real time PCR machine, different buffer systems, like the PACT screen can be
evaluated within an hour to find out the buffer in which your protein is
most stable.
It gives the Tm of your protein and if you have a high fluores
