, 2013 at 5:03 PM, Shiva Bhowmik gene1...@gmail.com wrote:
Dear All,
I am looking for references and/or example of substrate or ligand induced
oligomerization of enzymes related to activation.
Any help in this regard would be greatly appreciated.
Thanks.
Shiva
Dear All,
I am looking for references and/or example of substrate or ligand induced
oligomerization of enzymes related to activation.
Any help in this regard would be greatly appreciated.
Thanks.
Shiva
Dear All,
Wish you all a very happy new year. In this new year working on a problem
that I would like get your opinion.
Currently working on an enzyme that turnovers Coenzyme A (CoA)-conjugated
substrate. I have determined the structure of the enzyme and the structure
suggests to be a single
Hi Anna,
Interesting assembly. What is the function of your protein? Is it known if
your protein forms a fibril-like assembly in solution? Moreover, can your
crystal packing be indexed higher symmetry space group?
Cheers,
Shiva
On Mon, Jun 18, 2012 at 9:03 AM, David Schuller dj...@cornell.edu
Dear CCP4-ers,
Sorry for the offline topic. I would like to bring this the following
petition:
Dear Colleagues,
I wanted to bring your attention to a petition (see link below) started at
WhiteHouse.govhttp://WhiteHouse.gov/ by our colleague Dr. Steve Meltzer
at Johns Hopkins that calls for an
Dear CCP4-users,
Thank you all for your suggestions. Below is the compiled list of
suggestions for Iphone apps to display models:
1. Import structure directly from itunes in pdb format view in iMolview
(not free).
2. Cuemol for iOS: http://itunes.apple.com/us/**app/cuemol/id496236710?ls=1;
Dear All,
I was wondering if there is any IOS5 based app to display protein models,
which are not in public database, on Iphone. There is an app called
Molecules for displaying models but that utilizes coordinates from RCSB and
pubchem.
Thanks,
Shiva
Would be curious to know the current limitations on UV microscopy employed
for screening protein crystals - such as content of aromatic amino acids,
protein size etc.
Cheers,
Shiva
On Fri, Sep 16, 2011 at 1:19 AM, Klaus Fütterer k.futte...@bham.ac.ukwrote:
From the experience when our
Hi Ruheng,
Curious to know what was in the crystallization cocktail, cryoprotectant?
Did you collect the dataset at a synchrotron?
Shiva
2011/7/4 ruheng rh_ibp2...@hotmail.com
Dear all,
Recently we are working on an archaebacteria protein which was expressed
and purified from *E.coli *by
Of
*Shiva Bhowmik
*Sent:* Friday, June 24, 2011 11:42 AM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* Re: [ccp4bb] Y-Chi2 running out of chart
Hi Bie,
Curious to know what are the cell parameters obtained after scaling? You
mention observing perfect Chi2 statistics with lysozyme crystals
Hi Bie,
Curious to know what are the cell parameters obtained after scaling? You
mention observing perfect Chi2 statistics with lysozyme crystals. But are
you observing the same Chi2 statisctis with the crystal that yielded unusual
Y-Chi2 if you collect another dataset. If there is a consistency
Amonium sulfate precipitation? Considering the fact that you tried to
seperate tubulin contamination both by charge and mass difference amonium
sulfate pption may work.
Shiva
On Thu, Jun 23, 2011 at 4:05 AM, Seungil Han shan06...@gmail.com wrote:
All,
I am sorry that this is off topic.
My
Dear All,
I am working on a protein structure that yielded comparable diffraction
quality crystals from two different crystallization condition. One of the
crystallization condition conatins high conc. of salt pptant whereas the
oher one contains high conc. of organic pptant. There are some
Hi Cedric,
I presume you collected the datset at a synchrtron source. It could be free
radical generated due to X-ray radiation has modified the glutamate
residues. I have seen this happening for Ser residues.
Cheers,
Shiva
On Wed, May 18, 2011 at 9:08 AM, cedric bauvois cbauv...@gmail.com
? Acetylated perhaps ?
Jürgen
On May 18, 2011, at 1:56 PM, Shiva Bhowmik wrote:
Hi Cedric,
I presume you collected the datset at a synchrtron source. It could be free
radical generated due to X-ray radiation has modified the glutamate
residues. I have seen this happening for Ser residues
Hi All,
I also have a similar observation for proteins purified by Ni-NTA column. After
concentrating the sample eluted from the Ni-NTA column, I see a
brownish-yellowish tinge closer to the bottom of the filter with colorless
buffer on top. This is observed even for non-metal binding and
That's a possibility. Do you know if the same coloration would arise if there
is TCEP instead of DTT or b-ME? I usually have TCEP as the reducing agent.
Thanks Fillip.
Cheers,
Shiva
--- On Fri, 9/24/10, Filip Van Petegem filip.vanpete...@gmail.com wrote:
From: Filip Van Petegem
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