Hi, Phoebe,
Sorry to jump in late on this one- but I second Stefan's note here. When
soaking dinucleotides (which are poor substrates) into Klenow Fragment xtals, I
noted binding both at the active site and at a crystal interface site that is
likely nonphysiological. The adventitious site is
A biochemist friend asked for examples of cases were a protein was
co-crystallized with or soaked in a ligand that bound in the wrong place -
say, because the ligand used wasn't quite the right one or because other
important ligands were absent.
I'm sure such examples are out there, especially
