Dear Min,
regarding #1 some things come to my mind:
- What is the Kd that you got from the Biacore? And did you make sure that
the sample is concentrated enough (both on gel filtration and in
crystallization) to have a "sufficient" amount in the complex.
- did you use the same buffer systems?
with
to co-crystallize?
-Yes, I use the same buffer system for both proteins and complex.
Thank you all for your suggestions.
Min
Date: Fri, 16 Sep 2011 09:13:46 +0200
From: alexander.paut...@boehringer-ingelheim.com
Subject: [ccp4bb] AW: [ccp4bb] crystallization of complex and ...
To: CCP4BB
