Dear H.Sin,
These references may help you:
Meireles, M., Aimar, P., and Sanchez, V. (2004) Albumin denaturation during
ultrafiltration: effects of operating conditions and consequences on membrane
fouling, Biotech. Bioeng. 38, 528-534.
Schratter, P., (2004) Purification and concentration by
@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] BSA as additive
I haven't used BSA, but I did recently use 50mM L-glutamic acid for this exact
reason (and 5% glycerol in all buffers except the last one for crystallography)
after reading this paper and it made a big difference to my last protein prep:
https
C.UK
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference
where BSA has been used as an "additive" in the protein concentration step to
prevent aggregation of the main protein?
I would appreciate your suggestions.
Thank you,
H.Sin
bulletin board <CCP4BB@JISCMAIL.AC.UK> on behalf of Ha Sin
<haab...@gmail.com>
Sent: 08 May 2017 13:32:44
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference
where BSA has been used as
on behalf of Ha Sin
<haab...@gmail.com>
Sent: 08 May 2017 13:32:44
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] BSA as additive
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a reference
where BSA has been used as an "additive" in the protein
Dear CCP4BB members,
Sorry about my non crystallography question. Does anyone know of a
reference where BSA has been used as an "additive" in the protein
concentration step to prevent aggregation of the main protein?
I would appreciate your suggestions.
Thank you,
H.Sin
Dear All,
I am trying to calculate BSA (Buried surface area) for a protein complex. I am
using CCP4 AreaIMol.
I used Accessible surface area module and calculate areas for protein atoms
only
I know that the buried surface area (BSA) can be calculated as: BSA = ASA(A) +
ASA(B) - ASA(AB)
You should take a look at DIFFMODE COMPARE keyword in AREAIMOL manual,
it allows for buried surface area calculation (you have to prepare the
two pdb files with complex and receptor only). If you can easily use
command line and scripts (i.e. you are running CCP4 on non-micro$oft
OS), try the
PISA's interface area is 1/2 of sum BSAs for the interfacing
structures. If you go to
interface details, you also get total area of structures and their
individual BSAs.
The latter I should think would be an overinterpretation if used.
Eugene.
On Wed, May 26, 2010 at 1:45 PM, Sollepura Yogesha
