Re: [ccp4bb] Biolayer Interferometry

2014-10-10 Thread Cheng Yang
Sent via the Samsung GALAXY S®4 Active™, an AT&T 4G LTE smartphone Original message From: Alfredo Torres Date:2014/10/10 10:08 (GMT-06:00) To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Biolayer Interferometry Dear all, Sorry for the non-CCP4 question. We are evaluating

Re: [ccp4bb] Biolayer Interferometry

2014-10-10 Thread Jurgen Bosch
Whatever you decide to do, it is good practice to confirm your “hit” with an orthogonal method. Interactions in both directions with the same piece of equipment is a start but that in my eyes does not count as another method. If you are trying to decide between the Blitz and an Octet I would def

[ccp4bb] Biolayer Interferometry

2014-10-10 Thread Alfredo Torres
Dear all, Sorry for the non-CCP4 question. We are evaluating the current techniques to measure target-ligand interactions, and we would very much like to know the experience that the members of this community have about the BLItz/Octet system from ForteBio, in particular the rate of false pos