Thanks Eleanor,
I ended up using a glucose ligand lib file from a different protein that my
friend used and refinement Refmac5 worked out.
However I need to add the LINK Command in PDB so CCP4 recognizes that glucoses
connected.
Can I get help please?
Thank you,
Remie
On Mar 5, 2014, at
Have you run refmac with the Review restraints option - that generates
LINKs if they seem necessary.
Or can you use the SUGAR links already set upin the library?
Eleanor
On 7 March 2014 11:51, Remie remiefa...@gmail.com wrote:
Thanks Eleanor,
I ended up using a glucose ligand lib file from
On 07/03/14 11:51, Remie wrote:
I ended up using a glucose ligand lib file from a different protein
that my friend used and refinement Refmac5 worked out.
The mind boggles :)... well, OK.
However I need to add the LINK Command in PDB so CCP4 recognizes that
glucoses connected.
In
Hi,
I am trying to refine (Refmac5 on CCP4) a protein structure containing
ligands that are exclusively glucose units but refinement fails because I
don't have a lib file for the sugars. Can I get help on making lib files
please?
Thank you,
Remie
There are lots of GLUCOSE type lib files for different sugars.
One way to find what is available is:
From the REfinement GUI click Monomer library sketcher
You get a window with File in the top LH corner
Click on that and ask for Read monomer from library
Then enter a key word GLUCOSE and you
