Sounds odd as cross-linked resins should not exhibit such behaviour. I
have seen this with e.g. phosphocellulose columns. The charged groups on
the matrix repel each other at low ionic strength and expand hence the
increased bed volume with water etc. At high ionic strength the charges
are shield
Yes, CM-Sephadex expands and contracts incredibly. For other than batch methods
you will be much better using CM-Sepharose. I guess it is cross-linked
sepharose, and doesn't swell up much even in distilled water.
What is happening is the negative charges of the carboxy groups repel each other,
Hello All
I am using CM Sephadex C50 column for my protein. I equilibrated column with
MOPS buffer pH 6.0. But during elution I am using only NaCl2. This looks like
changing the bed height. Before starting elution the bed height was around
60cm. After starting elution the bed height decreased a
