[ccp4bb] Can I add PEG or Li2SO4 into protein sample for new screen?

2013-06-03 Thread Qing Shi
Hi everyone, I wonder if I can add PEG or Li2SO4 into protein sample for new screen? Because optimization seems no good result, initial crystal was got in Bis-Tris, PEG8000, Li2SO4. If it is OK, then what's the concentration of PEG or Li2SO4? Best regards, Shiqing

Re: [ccp4bb] Can I add PEG or Li2SO4 into protein sample for new screen?

2013-06-03 Thread Evgeny Osipov
Hi, Shiqing, In my opinion, it is not good idea. If you want to optimize the protein buffer than you could look at thermofluor method of protein buffer optimization. BTW, have you tried seeding? 03.06.2013 10:56, Qing Shi ?: Hi everyone, I wonder if I can add PEG or Li2SO4 into protein