Hi everyone,
I wonder if I can add PEG or Li2SO4 into protein sample for new screen?
Because optimization seems no good result, initial crystal was got in
Bis-Tris, PEG8000, Li2SO4. If it is OK, then what's the concentration of
PEG or Li2SO4?
Best regards,
Shiqing
Hi, Shiqing,
In my opinion, it is not good idea.
If you want to optimize the protein buffer than you could look at
thermofluor method of protein buffer optimization.
BTW, have you tried seeding?
03.06.2013 10:56, Qing Shi ?:
Hi everyone,
I wonder if I can add PEG or Li2SO4 into protein