Thank you everyone for the detailed explanations/suggestions and sharing
your successful experience. It is very helpful.
Earlier I had thought that maybe with ΔTm, I could select the most
promising molecules. But now it is unlikely the case.
I should have also mentioned that the IC50 values for a
*Dear Saif*
*Hope you are doing well and safe!*
1) How much change in Tm (ΔTm) in a thermal shift assay is considered to be
significant ?
*As it has already been mentioned there is no specific cutoff for deltaTm
to be considered significant. DeltaTm depends on many factors, including
the type o
Hi Saif,
If your goal is to perform co-crystallization, I am completely agreed with
David's suggestions. Delta Tm does matter in the co-crystallization but
that's not always the case. I have some experience with protein complexes
where I successfully co-crystallized by using really high molar rat
Hello,
we had an interesting case in the lab many years ago... Using the
shift-assay, a student managed to identify conditions that markedly
stabilized the protein of interest. To cut a long story short, in the
end it turned out conditions were identified that gave monomers while
the biologic
ee MX representative
==
about.me/david_briggs
From: CCP4 bulletin board on behalf of Saif Mohd
Sent: 25 February 2021 14:55
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Co-crystallization and thermal shift assay
Hello everyone,
1) How much change in Tm (ΔTm
Hello everyone,
1) How much change in Tm (ΔTm) in a thermal shift assay is considered to be
significant ?
2) A negative ΔTm infers that the compound is making the protein unstable.
In such a case, will the co-crystallization be difficult or just impossible
or on the contrary it shouldn't matter
