Re: [ccp4bb] Complex degradation

2009-05-25 Thread Herman . Schreuder
-Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Office 2004 Test Drive User Sent: Thursday, May 21, 2009 3:02 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Complex degradation Dear members, I am trying to crystallize a complex of two proteins

[ccp4bb] Complex degradation

2009-05-21 Thread Office 2004 Test Drive User
Dear members, I am trying to crystallize a complex of two proteins prepared in Tris, NaCl and DTT. The problem is one of the proteins degrades over time and allows only the second protein to crystallize. While addition of protease inhibitors such as EDTA is one of the ways to go, I would like to kn