-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Office 2004 Test Drive User
Sent: Thursday, May 21, 2009 3:02 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Complex degradation
Dear members,
I am trying to crystallize a complex of two proteins
Dear members,
I am trying to crystallize a complex of two proteins prepared in Tris, NaCl
and DTT. The problem is one of the proteins degrades over time and allows
only the second protein to crystallize. While addition of protease
inhibitors such as EDTA is one of the ways to go, I would like to kn