On 04/07/2014 03:16 PM, Eugene Valkov wrote:
Thanks for the input, Eugene. No, these do not impact phasing or
refinement significantly. We've SAD phased data like these with 5-Br and
5-I dU with no problems. The refinement is a bit tricky for some of the
structures because they are lower resol
nuing on the diffuse streak theme
Paul,
The fibre-like characteristics present in some diffraction patterns of nucleic
acids, which I think you are referring to, is the contribution of the helical
transform of the double-stranded helix. Essentially, the length of the regular
spacings in the base
Paul,
The fibre-like characteristics present in some diffraction patterns of
nucleic acids, which I think you are referring to, is the contribution of
the helical transform of the double-stranded helix. Essentially, the length
of the regular spacings in the base pairs modulates the intensities of
Seeing the diffuse streaking threads that have cropped up recently got
me to thinking about many of the DNA data sets we have collected on. We
always see diffuse streaking along lattice lines.
See images:
http://vespa.chem.umd.edu/~paul/hex_streak01.png
http://vespa.chem.umd.edu/~paul/hex_stre
