Hi Chen,
I had a similar condition before (reservoir solution with 10% dioxane) and an
oil-based cryoprotectant (66.5% w/w paraton-N, 28.5% paraffin oil and 5%
glycerol) worked for me. Please check this paper out:
http://scripts.iucr.org/cgi-bin/paper?wd5052
In addition, have you tried cryoprot
igher
viscosity you may not have to "chase your crystals" through the drop.
Hope this helps a little.
Mark
-Original Message-
From: Chen Zhao mailto:c.z...@yale.edu>>
To: CCP4BB mailto:CCP4BB@JISCMAIL.AC.UK>>
Sent: Wed, Jan 22, 2014 11:00 pm
Subject: [ccp4bb]
ion of the dioxane will still be an issue, but with a
> higher viscosity you may not have to "chase your crystals" through the
> drop.
>
> Hope this helps a little.
>
> Mark
>
>
>
> -Original Message-----
> From: Chen Zhao
> To: CCP4BB
> Sent:
ssage-
From: Chen Zhao
To: CCP4BB
Sent: Wed, Jan 22, 2014 11:00 pm
Subject: [ccp4bb] Control the crystallization process in the presence of small
volatile organic molecules
Dear all,
I am now optimizing a hit which contains about 30% 1,4-dioxane using
hanging-drop vapor diffusion at 25 degr
Dear all,
I am now optimizing a hit which contains about 30% 1,4-dioxane using
hanging-drop vapor diffusion at 25 degree. I am having a hard time to
reproduce the results: most of the times the drops are either dry in one
day or full of precipitate, and only occasionally could I get small
crystals
