I will try that. But I don't think people can tell the final
concentration of glutaraldehyde in the drop by using its vapor. Maybe
using direct soaking is what I should do. By the way, should I quench
it by ammonium or not?
Nian Huang
Dept of Biochemistry
UT Southwestern Medical Center
Dallas, TX
Nian,
It is also important to point out that gluteraldehyde is quite
penetrating (which is why it is used as a fixative in EM) and
volitile. A 1% solution is 100 mM, which is quite concentrated.
Adding gluteraldehyde by vapor diffusion is quite effective and
gentle, but does take a bit
Quoting Nian Huang [EMAIL PROTECTED]:
I will try that. But I don't think people can tell the final
concentration of glutaraldehyde in the drop by using its vapor. Maybe
using direct soaking is what I should do. By the way, should I quench
it by ammonium or not?
Nian Huang
Dept of Biochemistry
I used glutaraldehyde vapor by placing crystal drop above a bridge
with 15% glutaradehyde for various amount of time. It worked for me
for many crystals, but not for the one that I am trying now. The
crystal didn't diffract any more. I intended to make the crystal
tougher for heavy metal soaking.
Dear all,
I know most people use glutaraldehyde as the crosslinking reagent for
their crystals. But there are a lot of other crosslinking chemicals
available both in protein chemistry and histology, such as simple
formaldehyde. Did anybody have experience with other chemicals and how
did they
