Hi Brenda,
Maybe you want to have a look at the cryoprotectant database for
protein crystals.
http://idb.exst.jaxa.jp/db_data/protein/search-e.php
There you can search for your precipitant and get a list of
compatible freezing conditions that worked before including the
reference. It is
P4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprotectants for crystals
growing
in > 3M ammonium sulfate.
Thanks!
Brenda
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E-mail message checked by Spyware Do
bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Schulman, Brenda
Sent: Wednesday, August 19, 2009 6:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprotectants for crystals growing
in > 3M a
bulletin board [ccp...@jiscmail.ac.uk] On Behalf Of Savvas Savvides
[savvas.savvi...@ugent.be]
Sent: Wednesday, August 19, 2009 11:47 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hi Brenda
Try > 3M ammonium sulfate itself! We have tried that with
ulman, Brenda
Sent: Wednesday, August 19, 2009 12:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprotectants for crystals
growing
in > 3M ammonium sulfate.
Thanks!
Brenda
Email Disclaimer: www
-Original Message-
From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of
Schulman, Brenda
Sent: Wednesday, August 19, 2009 6:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Cryoprotection in > 3M ammonium sulfate
Hello!
I would be grateful for suggestions on cryoprot
Dear Brenda
Check out malonate
http://www.ncbi.nlm.nih.gov/pubmed/14646118?
ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubm
ed_DefaultReportPanel.Pubmed_RVDocSum
Malonate: a versatile cryoprotectant and stabilizing solution for
salt-grown macromolecular crystals.
H
Soluble possibilities include glucose, ethylene glycol, DMSO, glycerol
(15-30%). You may not need much cryoprotectant at all at these salt
concentrations. PEGs are not very soluble in high ammonium sulfate
solutions. (I think PEG-400 can go up to only 4% or so at 2 M ammonium
sulfate.)
Cheers
Hi Brenda,
You can try sugars like glucose, trehalose and sucrose for high AS contents.
It has been succesfully used in really hard cases such at protein RNA
crystals grown in AS. see Acta Cryst (2002) D58 1664-1669 Garber et al.
HTH
Pascal Egea
Hello!
I would be grateful for suggestions on cryoprotectants for crystals growing
in > 3M ammonium sulfate.
Thanks!
Brenda
Email Disclaimer: www.stjude.org/emaildisclaimer
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